Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Stereochemically-pure 2'-O-(2-methoxyethyl)-phosphorothioate (PS-MOE) oligonucleotides were synthesized from new chiral oxazaphospholidine-containing nucleosides. Thermal stability studies showed that the incorporation of Rp-PS linkages increased RNA-binding affinity. In cells, a full Rp-PS-MOE splice-switching oligonucleotide targeting part of the ferrochelatase gene was more potent than its Sp-PS counterpart, but of similar potency to the stereorandom PS-parent sequence.

Original publication

DOI

10.1039/c6cc08473g

Type

Journal article

Journal

Chem Commun (Camb)

Publication Date

03/01/2017

Volume

53

Pages

541 - 544

Keywords

Animals, Apolipoproteins B, Base Sequence, COS Cells, Chlorocebus aethiops, Ferrochelatase, Humans, Oligonucleotides, Antisense, Phosphorothioate Oligonucleotides, RNA, Messenger, Stereoisomerism