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Owing to the relationship between extracellular vesicles (EVs) and physiological and pathological conditions, the interest in EVs is exponentially growing. EVs hold high hopes for novel diagnostic and translational discoveries. This review provides an expert-based update of recent advances in the methods to study EVs and summarizes currently accepted considerations and recommendations from sample collection to isolation, detection, and characterization of EVs. Common misconceptions and methodological pitfalls are highlighted. Although EVs are found in all body fluids, in this review, we will focus on EVs from human blood, not only our most complex but also the most interesting body fluid for cardiovascular research.

Original publication

DOI

10.1161/CIRCRESAHA.117.309417

Type

Journal article

Journal

Circ Res

Publication Date

12/05/2017

Volume

120

Pages

1632 - 1648

Keywords

cardiovascular diseases, exosomes, extracellular vesicles, methods, reference standards, Biomarkers, Blood Specimen Collection, Cardiovascular Diseases, Exosomes, Extracellular Vesicles, Flow Cytometry, Humans